الخلاصة:
The study was structured around two main axes: on the one hand, a chemotaxonomic analysis
based on the similarity coefficients of Dice and Ochiai; on the other hand, a molecular
approach relying on the 16S rRNA gene sequence. It conducted on thirteen species from the
phylum Actinomycetota, all belonging to the genus Microbispora, namely: M. rosea, M.
catharanthi, M. saimensis, M. hainanensis, M. bryophytorum, M. triticiradicis, M. camponoti,
M. soli, M. sitophila, M. clausenae, M. oryzea, M. maris, and M. corallina. The objective of
the study was to conduct a comparative analysis among Microbispora species, and to identify
the species most closely related to Microbispora maris (considered the reference), through a
comparison of chemotaxonomic and molecular analysis results. For this purpose, the Ochiai
and Dice coefficients were applied to data derived from the chemical composition of the cell
walls, while bioinformatic algorithms were used to align nucleotide sequences and estimate
genetic distances. The results revealed a partial discrepancy between the two approaches. The
chemotaxonomic analysis indicated that M. catharanthi was the closest species to M. maris,
whereas the molecular analysis identified M. rosea as the most closely related species. This
study confirms that the two approaches are complementary for taxonomic classification
purposes.
