Abstract:
This study aims to perform a genomic analysis of the whole genome of an actinobacterial
strain isolated from a Saharan region of Algeria. The study allow us identify specific genes
that play an important role in enhancing plant growth and providing protection against
disease-causing agents. In this study, an actinobacterial strain called Saccharothrix
algeriensis DSM 44581 isolated from a soil sample collected from palm grove in Adrar
province, Algeria. The genomic classification analysis was conducted on the TYGS digital
platform (16S RNA) and phylogenomic (whole genome). The general features analyses are
conducted in DFAST, including the total length genome, GC % and CODs. Genomics
analysis of this strain focused on studying genes associated with functional gene categories
(COGs), this analysis involved genetic annotation using the RAST server and BV-BRC for
circular genome view, followed by gene prediction using the Prokka program in GALAXY to
identify genes involved in mechanisms that promote plant growth. In addition, the
antiSMASH involved genes related to the reduction of stress, such as ACC-deaminase and
various forms of osmoprotection such as glutathione, as well as several siderophores (YecD
and YclQ), were also emphasized. Genes such as DhbC and RecX are involved in the
biosynthesis of plant growth substances. The strain exhibits exceptional adaptation to
extremely salty settings. In addition, we have identified numerous genes that may be linked to
multiple enzymatic antioxidant processes. These mechanisms can reduce the excessive
formation of reactive oxygen species caused by salt stress. Genes involved in VOCs
production/degradation (e.g IlvB1 and AcuC), Quorum sensing (SsgB, kDa and Soj) and
phytohormones production/modulation (e.g PotB, PuuP and gabT) were reported. The
comparison studies of summary statistics in the Roary program (GALAXY) confirmed the
results of phylogenetic and phylogenomic studies. The extensive results demonstrate that
strain S. algeriensis DSM 44581 holds significant importance as a highly promising choice
for enhancing plant development and in biotechnological applications.
