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Étude de comparaison moléculaire et chimiotaxonomique (basée sur les coefficients de Dice et Ochiai) du genre Saccharomonospora

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dc.contributor.author LAHRECHE, Ikram
dc.contributor.author METTAS, Hakima
dc.contributor.author ZAGHLAOUI, Dalia Amina
dc.date.accessioned 2023-10-01T09:52:31Z
dc.date.available 2023-10-01T09:52:31Z
dc.date.issued 2023
dc.identifier.uri https://dspace.univ-ghardaia.edu.dz/xmlui/handle/123456789/6439
dc.description.abstract This study was conducted to investigate the taxonomic relationships between fourteen species of actinobacteria belonging to the genus Saccharomonospora. This study used two different approaches: a chemotaxonomic approach based on Dice and Ochiai coefficients, and a molecular approach based on the analysis of 16S ribosomal RNA. The species studied included S. amisosensis, S. azurea, S. colocasiae, S. cyanea, S. glauca, S. halophila, S. iraqiensis subsp. iraqiensis, S. iraqiensis subsp. paurometabolica, S. marina, S. oceani, S. piscinae, S. saliphila, S. viridis, S. xiaoerkulensis, and S. xinjiangensis. The objective of this study is to compare species of the genus Saccharomonospora using chemotaxonomy and molecular biology methods in order to determine those closest and furthest to the Saccharomonospora piscinae (selected as reference species) based on their degree of similarity. The analysis was performed using two statistical indices, Dice and Ochiai, with data for the chemical analysis of the species cellular composition obtained through a chemical analysis used for the chemotaxonomic study. As for the molecular study, it relies on bioinformatics algorithms that allow for the alignment of nucleotide sequences to identify similarities between species. Furthermore, the results of the study, using the Dice and Ochiai coefficients, revealed that S. ociani was the closest species to S. piscinae, followed by S. xiaoerkulensis, S. halophila, S. marina...etc. Additionally, the use of 16S rRNA sequences revealed that S. azurea and S. xinjiangensis were the two closest species to S. piscinae. The study has identified the furthest species, namely S. amisosensis and S. cyanea (for the chemotaxonomic study), and S. halophila and S. viridis (for the molecular study). Thus, these two analyses produced non-identical results EN_en
dc.language.iso fr EN_en
dc.publisher Faculté Science de la Nature et de la Vie et Sciences de la Terre - Université de Ghardaïa EN_en
dc.subject Saccharomonospora, chemotaxonomy, molecular study, Index of Dice and Ochiai, similarity, Saccharomonospora piscinae. EN_en
dc.subject Saccharomonospora, chimiotaxonomie, étude moléculaire, coefficients de Dice et Ochiai, similarité, Saccharomonospora piscinae EN_en
dc.title Étude de comparaison moléculaire et chimiotaxonomique (basée sur les coefficients de Dice et Ochiai) du genre Saccharomonospora EN_en


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