Molecular and chemotaxonomic comparative study (based on Jaccard’s and Kulczynski’s coefficients) of the genus Saccharomonospora

Abstract

A comparative study between the chemotaxonomy (based on Jaccard and Kulczynski coefficients') and molecular phylogeny based on the 16S rRNA gene sequences, of 15 species and subspecies of the genus Saccharomonospora, was carried out. The objective of this study is to deduce the evolutionary distances between the studied species and subspecies in reference to Saccharomonospora piscinae by the chemotaxonomic and molecular approaches, and work out the consistency between the 2 approaches. Chemical analysis results(mainly cellar wall components) were retrieved from the literature, analysed statistically using Jaccard’s and Kulczynski’s coefficients, and similairy orders were established accordingly. In addition, dendograms were constructed based on the Neighbour Joining method using Past4.12b software. The phylogenetic distances were obtained from the phylogetic tree based on the 16S rRNA gene sequences using MEGA11 software, based on the Neighbour Joining method. The results showed a consistanty between the molecular approach using Neighbour Joining method and the chemotaxonomy based on the coefficient of Jaccard, while both coefficients exhibited an overall similar dendogram topology, eventhough the similarity order was not completly the same. In conclusion, due to the descripencies in the chemotaxonomic study, the results should be taken reservedly, and more statistical coeffiecients should be investigated in the future. The molecular study based on 16S rRNA and whole genom approache still offer far better tool for the taxonomy of Saccharomonospora at the species and subspecies levels.